Tryptophan

29 research sources Also known as: l-tryptophan tryptophan supplement
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500.0 mg Median Dose
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29 Research Sources
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Evidence Summary

L-Tryptophan is an essential amino acid and a precursor to serotonin, primarily studied for its role in regulating sleep, mood, and the kynurenine pathway. Research indicates potential benefits for sleep quality and mood regulation, though evidence regarding its efficacy as a standalone treatment for depression is mixed.

Evidence by Condition

Sleep Moderate

Supplementation, particularly when combined with ingredients like mulberry leaf extract, has shown potential to improve sleep quality and sleep initiation.

Depression Moderate

Meta-analyses have investigated tryptophan and 5-HTP as natural alternatives to traditional antidepressants, but results are varied.

Research explores associations between tryptophan levels and postpartum mood disorders, as well as the effects of acute tryptophan depletion on mood.

Gut Health Weak

Tryptophan metabolism is closely linked to the gut microbiome and the kynurenine pathway, influenced by prebiotics and probiotics.

Effective Doses

Dose ranges are not explicitly stated in the provided research studies.

Key Findings

  • L-tryptophan supplementation has been assessed for its positive effect on sleep quality (PMID: 33942088).
  • Tryptophan and 5-hydroxytryptophan have been studied as natural alternatives for treating unipolar depression and dysthymia (PMID: 11687048).
  • A combination of mulberry leaf extract and tryptophan can improve sleep and post-wake mood in adults (PMID: 40072601).
  • There is a documented association between tryptophan levels and postpartum mood disorders (PMID: 35941560).

Limitations

Much of the existing research relies on acute tryptophan depletion models rather than long-term supplementation, and there is a lack of standardized dosing data across clinical trials.

Safety & Interactions

Changes in tryptophan metabolism affect the kynurenine pathway, which can impact the immune system and overall human physiology (PMID: 34114978).

AI-generated summary from 29 peer-reviewed studies. Not medical advice. Always consult a healthcare provider.

Claims vs. Evidence

100% Evidence-Backed
0% Unsupported
90% Structure/Function
Claim Type% of ProductsEvidence RequiredStatus
All Other 100% Tier D (Unclassified) ✅ Supported
Structure/Function 90% Tier C (DSHEA Structure/Function (no pre-approval)) ✅ Supported
Nutrient 61% Tier D (Unknown) ✅ Supported

Research by Topic

Research Evidence (29)

Rct: 19 Meta Analysis: 5 Systematic Review: 3 Clinical Trial: 2
B Prebiotic and probiotic supplementation and the tryptophan-kynurenine pathway: A systematic review and meta analysis
Meta Analysis Neuroscience and biobehavioral reviews 2021 n=253

**Purton et al., 2021** | Neurosci Biobehav Rev | Meta Analysis Purton Terry, Staskova Lada, ... Marx Wolfgang. Prebiotic and probiotic supplementation and the tryptophan-kynurenine pathway: A systematic review and meta analysis. Neurosci Biobehav Re...

B Tryptophan and 5-hydroxytryptophan for depression
Systematic Review The Cochrane database of systematic reviews 2001 n=64

BACKGROUND: 5 Hydroxytryptophan (5-HTP) and tryptophan are so-called natural alternatives to traditional antidepressants, used to treat unipolar depression and dysthymia. OBJECTIVES: To determine whether 5-HTP and tryptophan are more effective than p...

B Tryptophan and 5-hydroxytryptophan for depression
Systematic Review The Cochrane database of systematic reviews 2002 n=64

BACKGROUND: 5 Hydroxytryptophan (5-HTP) and tryptophan are so-called natural alternatives to traditional antidepressants, used to treat unipolar depression and dysthymia. OBJECTIVES: To determine whether 5-HTP and tryptophan are more effective than p...

B The impact of tryptophan supplementation on sleep quality: a systematic review, meta-analysis, and meta-regression
Meta Analysis Nutrition reviews 2022

CONTEXT: L-tryptophan (Trp) has been documented to aid sleep, but a systematic compilation of its effect on sleep quality is still limited. OBJECTIVE: We assessed the effect of Trp supplementation on sleep quality via meta-analysis and meta-regressio...

B No effects of acute tryptophan depletion on anxiety or mood in weight-recovered female patients with anorexia nervosa
Rct European archives of psychiatry and clinical neuroscience 2023 n=22

BACKGROUND: Previous studies have suggested that individuals recovered from anorexia nervosa (AN) are characterized by increased serotonergic (5-HT) activity that might be related to elevated levels of anxiety. Assuming these traits to be also presen...

C The association between tryptophan levels and postpartum mood disorders: a systematic review and meta-analysis
Meta Analysis BMC psychiatry 2022

Over 50% of women experience mood disturbance in the postpartum period, with significant implications for maternal and infant health but identifying those at risk is not easily possible. The essential amino acid, tryptophan (TRP) through its neuroact...

C A systematic review of the effect of L-tryptophan supplementation on mood and emotional functioning
Systematic Review Journal of dietary supplements 2021 n=11

L-tryptophan (TRP), one of the essential amino acids in humans, is a precursor of serotonin, and hence its intake is closely related to the suppression of depressed and anxious moods. We did a systematic review of RCTs to examine the effects of trypt...

C Comparison between Acupuncture and Nutraceutical Treatment with Migratens® in Patients with Fibromyalgia Syndrome: A Prospective Randomized Clinical Trial.
Rct Nutrients

OBJECTIVES: Fibromyalgia syndrome (FMS) is a chronic clinical condition characterized by pain, fatigue, altered sleep, and cognitive disturbances. The purpose of this study was to compare two alternative treatments (nutraceutical and acupuncture) in ...

C Intake of Lactiplantibacillus plantarum HEAL9 Improves Cognition in Moderately Stressed Subjects: A Randomized Controlled Study.
Rct Nutrients

BACKGROUND: The usage of probiotics has expanded beyond the areas of gut and immune health improvement. Several studies have shown the positive impact associated between probiotics and stress, cognition, and mood; a relationship referred to as the gu...

C Are tryptophan and 5-hydroxytryptophan effective treatments for depression? A meta-analysis
Meta Analysis The Australian and New Zealand journal of psychiatry 2002 n=64

**Shaw et al., 2002** | Aust N Z J Psychiatry | Meta Analysis Shaw Kelly, Turner Jane, Del Mar Christopher. Are tryptophan and 5-hydroxytryptophan effective treatments for depression? A meta-analysis. Aust N Z J Psychiatry. 2002-Aug;36(4):488-91 OBJE...

C Predictors of mood response to acute tryptophan depletion. A reanalysis
Meta Analysis Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2002

Acute tryptophan depletion (ATD) induces depressive symptoms in 50-60% of selective serotonin reuptake inhibitor (SSRI) treated, recovered depressed patients. However, no reliable predictors of mood response to ATD have been established. In the prese...

C Two apples a day modulate human:microbiome co-metabolic processing of polyphenols, tyrosine and tryptophan.
Rct European journal of nutrition

PURPOSE: Validated biomarkers of food intake (BFIs) have recently been suggested as a useful tool to assess adherence to dietary guidelines or compliance in human dietary interventions. Although many new candidate biomarkers have emerged in the last ...

C Effect of aprepitant on kynurenine to tryptophan ratio in cART treated and cART naïve adults living with HIV.
Rct Medicine

Changes in tryptophan metabolism affect human physiology including the immune system, mood, and sleep and are associated with human immunodeficiency virus (HIV) pathogenesis. This study investigates whether the treatment of HIV-infected individuals w...

C The role of nutraceuticals in the management of temporomandibular disorders.
Rct Journal of complementary & integrative medicine

OBJECTIVES: Temporomandibular disorders (TMDs) are usually treated with occlusal appliances and supportive treatments such as physical therapy and drugs. Supplements can be included among potential supportive therapies, with the aim of reducing the u...

C Mulberry leaf extract combined with tryptophan improves sleep and post wake mood in adults with sleep complaints - A randomized cross-over study.
Rct European journal of nutrition

PURPOSE: In the current study we evaluated a blend of ingredients containing mulberry leaf extract (to lower postprandial glucose of the evening meal), tryptophan (facilitator of the sleep initiation) to benefit sleep initiation and quality in adults...

C Effect of melatonin enriched with L-Tryptophan and 5-Hydroxytryptophan on sleep parameters in children with neurodevelopmental disorders.
Rct Sleep medicine

INTRODUCTION: Melatonin plays a key role in sleep regulation. Combining melatonin with its precursors, L-tryptophan (LT) and 5-Hydroxytryptophan (5HTP), may influence sleep-related outcomes, but evidence in children with neurodevelopmental disorders ...

C Use of nutritional supplements based on melatonin, tryptophan and vitamin B6 (Melamil Tripto®) in children with primary chronic headache, with or without sleep disorders: a pilot study.
Rct Minerva pediatrica

BACKGROUND: Headache is one of the main complaints in pediatric neurology. Exogenous melatonin has been shown to be useful and safe in improving sleep-wake cycles and sleep quality in children. Tryptophan as well plays a key role in sleep regulation....

C Psychological and Sleep Effects of Tryptophan and Magnesium-Enriched Mediterranean Diet in Women with Fibromyalgia.
Rct International journal of environmental research and public health

Anxiety, mood disturbance, eating and sleep disorders, and dissatisfaction with body image are prevalent disorders in women with fibromyalgia. The authors of this study aimed to determine the effects of tryptophan (TRY) and magnesium-enriched (MG) Me...

C Presleep α-Lactalbumin Consumption Does Not Improve Sleep Quality or Time-Trial Performance in Cyclists.
Rct International journal of sport nutrition and exercise metabolism

We tested the hypothesis that presleep consumption of α-lactalbumin (LA), a fraction of whey with a high abundance of tryptophan, would improve indices of sleep quality and time-trial (TT) performance in cyclists relative to an isonitrogenous collage...

C Evening Whey Protein Intake, Rich in Tryptophan, and Sleep in Elite Male Australian Rules Football Players on Training and Nontraining Days.
Rct International journal of sport nutrition and exercise metabolism

OBJECTIVES: To investigate the effect of evening whey protein supplementation, rich in tryptophan, on sleep in elite male Australian Rules Football players. DESIGN: Double-blinded, counterbalanced, randomized, cross-over study. METHODS: Sleep was ass...

C Infant growth and tolerance with a formula based on novel native demineralized whey: A randomized double-blind pilot study.
Rct Journal of pediatric gastroenterology and nutrition

OBJECTIVES: The aim of the study was to evaluate the effects on infant growth and tolerance of a Test infant formula based on a novel whey extraction and demineralization process, compared to a Standard formula and a breastfed reference arm. METHODS:...

C Evening Alpha-Lactalbumin Supplementation Alters Sleep Architecture and Reduces Morning Reaction Time in an Athletically Trained Population With Sleep Difficulties.
Rct International journal of sport nutrition and exercise metabolism

Evening consumption of a whey protein rich in the amino acid tryptophan, alpha-lactalbumin (ALAC), has previously shown to benefit sleep-particularly among poor sleepers. Given trained populations often experience sleep difficulty, this study investi...

C Minimal Influence of Formulated Nutritional Interventions on Sleep and Next-Morning Physical Performance, Cognitive Function, and Postural Sway in Adult Males: A Randomized, Placebo-Controlled, Crossover Study.
Rct International journal of sport nutrition and exercise metabolism

Athletes often experience poor sleep quality and quantity which may hinder physical performance and cognitive function. Presleep nutritional strategies may be an alternative to pharmacological interventions to improve sleep. The aim of this study was...

C Effects of Coenzyme Q10, Tryptophan, and Magnesium Supplementation on Fatigue in Patients with Fibromyalgia - A Randomized Trial.
Rct Journal of dietary supplements

Fibromyalgia (FM) is a prevalent and multifactorial condition requiring pharmacological and non-pharmacological interventions for its management. Coenzyme Q10 (CoQ10), magnesium, and tryptophan are associated with FM symptoms, but their combined effe...

C Daily walnut consumption increases 6-sulfatoxymelatonin urinary levels and can improve sleep quality: a randomized crossover trial.
Rct Food & function

We aimed to study the potential impact of daily consumption of walnuts on urinary 6-sulphatoxymelatonin (6-SMT) levels and sleep quality parameters. We conducted an open-label randomized crossover trial (NCT04799821) in 76 young adults (24.1 ± 3.4 ye...

C Walnut peptide and theanine combination ameliorates sleep disorders: a multi-species study including a human trial.
Rct Food & function

Chronic stress-induced sleep disorders are characterized by disrupted sleep architecture and a significant reduction in slow-wave sleep (SWS). These disorders represent a major public health challenge and are mechanistically linked to the hyperactiva...

C Short-term sleep restriction in humans alters diurnal circulating metabolite profiles, including those of microbial origin.
Rct The Journal of clinical investigation

BACKGROUNDGut microbes and their metabolites contribute to the host circulating metabolome and exhibit diurnal variation influenced by sleep-wake cycles and meal timing. Sleep deprivation alters the rhythmic circulating metabolome, but its impact on ...

C Tryptophan Metabolism in Human Brain Tumors-Novocure
Clinical Trial ClinicalTrials.gov 2014

**NCT ID**: NCT02367482 **Phase**: **Status**: COMPLETED **Enrollment**: 20 **Completion**: 2023-12-31

C The Effect of Cysteine, Tryptophan and Tyrosine Supplements on Breast Milk
Clinical Trial ClinicalTrials.gov 2015

**NCT ID**: NCT02462746 **Phase**: PHASE1 **Status**: COMPLETED **Enrollment**: 24 **Completion**: 2016-04

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Frequently Asked Questions

What is L-Tryptophan and how does it work?

L-Tryptophan is an essential amino acid that the body uses as a precursor to produce serotonin. It plays a key role in regulating sleep, mood, and the kynurenine pathway in the body.

Can L-Tryptophan help improve my sleep?

There is moderate evidence suggesting that L-Tryptophan can improve sleep quality and help you fall asleep more easily. Some research even indicates that combining it with mulberry leaf extract may improve both sleep and your mood after waking up.

Is L-Tryptophan effective for treating depression?

There is moderate evidence investigating L-Tryptophan as a natural alternative to traditional antidepressants, but the results are mixed. Because the evidence is varied, it should not be relied upon as a standalone treatment for depression.

Does L-Tryptophan help with anxiety or postpartum mood?

There is currently weak evidence regarding the use of L-Tryptophan for anxiety and mood regulation. While some studies have noted an association between tryptophan levels and postpartum mood disorders, more research is needed.

What is the recommended dose of L-Tryptophan?

The provided research does not explicitly state a specific effective dose range for supplementation. You should consult with a healthcare professional to determine an appropriate dose for your needs.

Are there any safety concerns or side effects with L-Tryptophan?

Changes in how your body processes tryptophan can affect the kynurenine pathway, which may impact your immune system and overall physiology. It is important to be aware of how supplementation might influence these biological processes.

Are there any drug interactions or people who should avoid L-Tryptophan?

No specific drug interactions have been documented for L-Tryptophan in the available research. However, because it can affect the immune system and metabolic pathways, you should consult a doctor before use, especially if you have underlying health conditions.

Answers generated from research evidence. Not medical advice.