Is Vitamin D Fortification of Dairy Products Effective for Improving Vitamin D Status? A Systematic Review and Meta-Analysis of Randomised Controlled Trials

source extracted confidence: high 2026-04-09
#adult #animals #female #humans #male

Is Vitamin D Fortification of Dairy Products Effective for Improving Vitamin D Status? A Systematic Review and Meta-Analysis of Randomised Controlled Trials

Wong et al., 2025 | Nutrients | Meta Analysis

Citation

Wong Cheuk Lun, Givens D Ian, ... Guo Jing. Is Vitamin D Fortification of Dairy Products Effective for Improving Vitamin D Status? A Systematic Review and Meta-Analysis of Randomised Controlled Trials. Nutrients. 2025-Nov-29;17(23). doi:10.3390/nu17233757

Abstract

Background/objectives: Given the suboptimal vitamin D intake and status among the United Kingdom population, the Scientific Advisory Committee on Nutrition is seeking suitable food vehicles for vitamin D fortification. Thus, this study aimed to examine the efficacy of vitamin D-fortified dairy products in improving serum 25-hydroxyvitamin D [25(OH)D] concentration using data from randomised controlled trials (RCTs). Methods: PubMed, Embase, and Web of Science were searched from inception until October 2024. Studies were included if they were RCTs with intervention groups administered vitamin D-fortified dairy products and control groups administered unfortified dairy products, as well as examining the effects on serum 25(OH)D concentration. Results: There were 35 RCTs eligible for inclusion, involving 4965 participants (intervention: 2526; control: 2439). The results showed that serum 25(OH)D concentrations were significantly increased by vitamin D3-fortified milk/milk powder (n = 15, mean difference (MD): 18.31 nmol/L, 95% confidence interval (CI): 13.30-23.33 nmol/L, I2 = 95%), vitamin D3-fortified yoghurt/yoghurt drinks (n = 11, MD: 26.22 nmol/L, 95% CI: 18.67-33.77 nmol/L, I2 = 97%), vitamin D2-fortified milk/milk powder (n = 3, MD: 11.61 nmol/L, 95% CI: 9.31-13.91 nmol/L, I2 = 0%), vitamin D-fortified (type not specified) milk/milk powder (n = 8, MD: 13.59 nmol/L, 95% CI: 8.54-18.64, I2 = 98%), and vitamin D-fortified (type not specified) yoghurt/yoghurt drinks (n = 4, MD: 27.74 nmol/L, 95% CI: 16.83-38.64 nmol/L, I2 = 91%), but insignificantly increased by vitamin D3-fortified cheese (n = 5, MD: 16.78 nmol/L, 95% CI: -3.61-37.16, I2 = 99%). However, the results of vitamin D3-fortified cheese became significant when leave-one-out analysis was performed by omitting one RCT (MD: 24.13 nmol/L, 95% CI: 4.69-43.58, I2 = 90%). Conclusions: These findings provide evidence that vitamin D-fortified dairy products have the potential to improve serum 25(OH)D concentrations in populations.

Key Findings

There were 35 RCTs eligible for inclusion, involving 4965 participants (intervention: 2526; control: 2439). The results showed that serum 25(OH)D concentrations were significantly increased by vitamin D3-fortified milk/milk powder (n = 15, mean difference (MD): 18.31 nmol/L, 95% confidence interval (CI): 13.30-23.33 nmol/L, I2 = 95%), vitamin D3-fortified yoghurt/yoghurt drinks (n = 11, MD: 26.22 nmol/L, 95% CI: 18.67-33.77 nmol/L, I2 = 97%), vitamin D2-fortified milk/milk powder (n = 3, MD: 11.

Outcomes Measured

  • Requires manual extraction

Population

Field Value
Population See abstract
Sample Size 15
Age Range See abstract
Condition See abstract

MeSH Terms

  • Adult
  • Animals
  • Female
  • Humans
  • Male
  • Middle Aged
  • Cholecalciferol
  • Dairy Products
  • Food, Fortified
  • Milk
  • Nutritional Status
  • Randomized Controlled Trials as Topic
  • Vitamin D
  • Vitamin D Deficiency

Evidence Classification

  • Level: Meta Analysis
  • Publication Types: Journal Article, Meta-Analysis, Systematic Review
  • Vertical: vitamin-d

Provenance

Source extracted via PubMed E-utilities API on 2026-04-09