Vitamin B12, folate, and homocysteine levels in children and adolescents with obesity: a systematic review and meta-analysis

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#humans #vitamin-b-12 #homocysteine #child #folic-acid

Vitamin B12, folate, and homocysteine levels in children and adolescents with obesity: a systematic review and meta-analysis

Ulloque-Badaracco et al., 2025 | Front Public Health | Meta Analysis

Citation

Ulloque-Badaracco Juan R, Alarcon-Braga Esteban A, ... Benites-Zapata Vicente A. Vitamin B12, folate, and homocysteine levels in children and adolescents with obesity: a systematic review and meta-analysis. Front Public Health. 2025;13:1481002. doi:10.3389/fpubh.2025.1481002

Abstract

BACKGROUND AND AIMS: Childhood and adolescent obesity is a global public health concern. Obesity induces several metabolic disturbances. Several studies have explored the association of vitamin B12, folate, and homocysteine (Hcy) with obesity. This study aimed to synthesize the available evidence regarding the differences in serum levels of vitamin B12, Hcy, and folate among children or adolescents with and without obesity. METHODS: A random-effects meta-analysis using the Sidik-Jonkman method and corrected 95% confidence interval (CI) using the truncated Knapp-Hartung standard errors was used for all meta-analyses. Standardized mean difference (SMD) with the corresponding 95% CI was used as the only effect size. The Cochran's Q test and the I2 statistic were used to evaluate between-study heterogeneity. Publication bias was assessed using funnel plots and the Egger test. RESULTS: Twenty studies were included with a combined study population of 7,791 patients. There were no significant differences between children/adolescents with and without obesity with respect to serum vitamin B12 levels (SMD: -0.24; 95% CI: -0.53 to 0.06; p > 0.05, I2 = 74.93%) and folate levels (SMD: -0.12; 95% CI: -0.29 to 0.06; p > 0.05, I2 = 19.6%). However, children/adolescents with obesity had significantly higher Hcy levels compared to counterparts without obesity (SMD: 0.77; 95% CI: 0.39 to 1.14; p < 0.001, I2 = 86.4%). CONCLUSION: Children and adolescents with obesity had higher Hcy levels than those without obesity. However, no significant differences were found for vitamin B12 and folate levels. Hcy may play a role in the development of obesity in this population.

Key Findings

Twenty studies were included with a combined study population of 7,791 patients. There were no significant differences between children/adolescents with and without obesity with respect to serum vitamin B12 levels (SMD: -0.24; 95% CI: -0.53 to 0.06; p > 0.05, I2 = 74.93%) and folate levels (SMD: -0.12; 95% CI: -0.29 to 0.06; p > 0.05, I2 = 19.6%). However, children/adolescents with obesity had significantly higher Hcy levels compared to counterparts without obesity (SMD: 0.77; 95% CI: 0.39 to 1.

Outcomes Measured

  • Requires manual extraction

Population

Field Value
Population See abstract
Sample Size 7791
Age Range See abstract
Condition See abstract

MeSH Terms

  • Humans
  • Vitamin B 12
  • Homocysteine
  • Child
  • Folic Acid
  • Adolescent
  • Pediatric Obesity
  • Female
  • Male

Evidence Classification

  • Level: Meta Analysis
  • Publication Types: Journal Article, Meta-Analysis, Systematic Review
  • Vertical: vitamin-b12-homocysteine

Provenance

Source extracted via PubMed E-utilities API on 2026-04-09