Inhibitory Effects of Probiotic and Gastro-Intestinal Bacteria on Helicobacter pylori in vitro

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#helicobacter-pylori #probiotics #humans #helicobacter-infections #gastrointestinal-microbiome

Inhibitory Effects of Probiotic and Gastro-Intestinal Bacteria on Helicobacter pylori in vitro

Westphal et al., 2025 | Digestion | Systematic Review

Citation

Westphal Johannes Raphael, Koch Nadine, ... Schulz Christian. Inhibitory Effects of Probiotic and Gastro-Intestinal Bacteria on Helicobacter pylori in vitro. Digestion. 2025;106(4):349-364. doi:10.1159/000543447

Abstract

INTRODUCTION: Helicobacter pylori is a highly prevalent pathogen affecting approximately 50% of the world population, causing chronic gastritis and subsequently adenocarcinoma. Antibiotic resistance rates in H. pylori are increasing, thus demanding alternative treatment options. Some beneficial bacteria, including probiotics and gastrointestinal commensals, were shown to inhibit H. pylori growth, viability, and initial attachment to the gastric epithelium. METHODS: In this review, we systematically summarized the currently available literature for in vitro inhibition of H. pylori through beneficial bacteria from the Lactobacillales order. We performed research on PubMed and Google Scholar in accordance with the PRISMA guidelines. RESULTS: A multitude of species were shown to possess anti-H. pylori activity, although the majority of investigated bacteria belonged to only one bacterial genus: Lactobacillus. Anti-H. pylori activity was mediated through transcriptional modulation of virulence factors, competition for binding sites, the induction of a dormancy state of H. pylori, and the secretion of anti-H. pylori compounds. CONCLUSION: Many bacterial compounds that show probiotic properties are capable of inhibiting H. pylori in in vitro experiments. However, a huge variety of test methods to detect anti-H. pylori effects demands standardization.

Key Findings

A multitude of species were shown to possess anti-H. pylori activity, although the majority of investigated bacteria belonged to only one bacterial genus: Lactobacillus. Anti-H. pylori activity was mediated through transcriptional modulation of virulence factors, competition for binding sites, the induction of a dormancy state of H. pylori, and the secretion of anti-H. pylori compounds.

Outcomes Measured

  • Requires manual extraction

Population

Field Value
Population See abstract
Sample Size See abstract
Age Range See abstract
Condition See abstract

MeSH Terms

  • Helicobacter pylori
  • Probiotics
  • Humans
  • Helicobacter Infections
  • Gastrointestinal Microbiome
  • Gastric Mucosa
  • Antibiosis
  • Lactobacillus

Evidence Classification

  • Level: Systematic Review
  • Publication Types: Journal Article, Systematic Review
  • Vertical: probiotics

Provenance

Source extracted via PubMed E-utilities API on 2026-04-09