Engineered production of bioactive polyphenolic O-glycosides

source extracted confidence: high 2026-04-09
#humans #glycosides #carbohydrates #glycosylation #glycosyltransferases

Engineered production of bioactive polyphenolic O-glycosides

Ren et al., 2023 | Biotechnol Adv | Systematic Review

Citation

Ren Jie, Barton Caleb Don, Zhan Jixun. Engineered production of bioactive polyphenolic O-glycosides. Biotechnol Adv. 2023;65:108146. doi:10.1016/j.biotechadv.2023.108146

Abstract

Polyphenolic compounds (such as quercetin and resveratrol) possess potential medicinal values due to their various bioactivities, but poor water solubility hinders their health benefits to humankind. Glycosylation is a well-known post-modification method to biosynthesize natural product glycosides with improved hydrophilicity. Glycosylation has profound effects on decreasing toxicity, increasing bioavailability and stability, together with changing bioactivity of polyphenolic compounds. Therefore, polyphenolic glycosides can be used as food additives, therapeutics, and nutraceuticals. Engineered biosynthesis provides an environmentally friendly and cost-effective approach to generate polyphenolic glycosides through the use of various glycosyltransferases (GTs) and sugar biosynthetic enzymes. GTs transfer the sugar moieties from nucleotide-activated diphosphate sugar (NDP-sugar) donors to sugar acceptors such as polyphenolic compounds. In this review, we systematically review and summarize the representative polyphenolic O-glycosides with various bioactivities and their engineered biosynthesis in microbes with different biotechnological strategies. We also review the major routes towards NDP-sugar formation in microbes, which is significant for producing unusual or novel glycosides. Finally, we discuss the trends in NDP-sugar based glycosylation research to promote the development of prodrugs that positively impact human health and wellness.

Key Findings

Finally, we discuss the trends in NDP-sugar based glycosylation research to promote the development of prodrugs that positively impact human health and wellness.

Outcomes Measured

  • Requires manual extraction

Population

Field Value
Population See abstract
Sample Size See abstract
Age Range See abstract
Condition See abstract

MeSH Terms

  • Humans
  • Glycosides
  • Carbohydrates
  • Glycosylation
  • Glycosyltransferases
  • Sugars
  • Nucleotides

Evidence Classification

  • Level: Systematic Review
  • Publication Types: Systematic Review, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.
  • Vertical: resveratrol

Provenance

Source extracted via PubMed E-utilities API on 2026-04-09