Raspberry Ketone Reduced Lipid Accumulation in 3T3-L1 Cells and Ovariectomy-Induced Obesity in Wistar Rats by Regulating Autophagy Mechanisms

source extracted confidence: high 2026-04-09
#3t3-l1-cells #adipocytes #adipogenesis #animals #anti-obesity-agents

Raspberry Ketone Reduced Lipid Accumulation in 3T3-L1 Cells and Ovariectomy-Induced Obesity in Wistar Rats by Regulating Autophagy Mechanisms

Leu et al., 2017 | J Agric Food Chem | Other

Citation

Leu Sy-Ying, Chen Yi-Chen, ... Cheng Pao-Yun. Raspberry Ketone Reduced Lipid Accumulation in 3T3-L1 Cells and Ovariectomy-Induced Obesity in Wistar Rats by Regulating Autophagy Mechanisms. J Agric Food Chem. 2017-Dec-20;65(50):10907-10914. doi:10.1021/acs.jafc.7b03831

Abstract

This study aimed to determine the antiobesity effects of raspberry ketone (RK), one of the major aromatic compounds contained in raspberry, and its underlying mechanisms. During adipogenesis of 3T3-L1 cells, RK (300 μM) significantly reduced lipid accumulation and downregulated the expression of CCAAT/enhancer-binding protein α (C/EBPα), peroxisome proliferation-activated receptor γ (PPARγ), fatty acid-binding protein 4 (FABP4), and fatty acid synthase (FAS). RK also reduced the expression of light chain 3B (LC3B), autophagy-related protein 12 (Atg12), sirtuin 1 (SIRT1), and phosphorylated-tuberous sclerosis complex 2 (TSC2), whereas it increased the level of p62 and phosphorylated-mammalian target of rapamycin (mTOR). Daily administration of RK decreased the body weight (ovariectomy [Ovx] + RK, 352.6 ± 5 vs Ovx, 386 ± 5.8 g; P < 0.05), fat mass (Ovx + RK, 3.2 ± 0.05 vs Ovx, 5.0 ± 0.4 g; P < 0.05), and fat cell size (Ovx + RK, 6.4 ± 0.6 vs Ovx, 11.1 ± 0.7 × 103 μm2; P < 0.05) in Ovx-induced obesity in rats. The expression of PPARγ, C/EBPα, FAS, and FABP4 was significantly reduced in the Ovx + RK group compared with that in the Ovx group. Similar patterns were observed in autophagy-related proteins and endoplasmic reticulum stress proteins. These results suggest that RK inhibited lipid accumulation by regulating autophagy in 3T3-L1 cells and Ovx-induced obese rats.

Key Findings

These results suggest that RK inhibited lipid accumulation by regulating autophagy in 3T3-L1 cells and Ovx-induced obese rats.

Outcomes Measured

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Population

Field Value
Population See abstract
Sample Size See abstract
Age Range See abstract
Condition stress

MeSH Terms

  • 3T3-L1 Cells
  • Adipocytes
  • Adipogenesis
  • Animals
  • Anti-Obesity Agents
  • Autophagy
  • Butanones
  • CCAAT-Enhancer-Binding Protein-alpha
  • Female
  • Humans
  • Lipid Metabolism
  • Mice
  • Obesity
  • Ovariectomy
  • PPAR gamma
  • Plant Extracts
  • Rats
  • Rats, Wistar
  • Rubus
  • Sirtuin 1
  • Tuberous Sclerosis Complex 2 Protein
  • Tumor Suppressor Proteins

Evidence Classification

  • Level: Other
  • Publication Types: Journal Article
  • Vertical: raspberry-ketones

Provenance

Source extracted via PubMed E-utilities API on 2026-04-12