Bone Healing via Carvacrol and Curcumin Nanoparticle on 3D Printed Scaffolds

source extracted confidence: high 2026-04-09
#cymenes #nanoparticles #tissue-scaffolds #curcumin #printing-three-dimensional

Bone Healing via Carvacrol and Curcumin Nanoparticle on 3D Printed Scaffolds

Dahiya et al., 2024 | Small | Other

Citation

Dahiya Aditi, Chaudhari Vishal Sharad, Bose Susmita. Bone Healing via Carvacrol and Curcumin Nanoparticle on 3D Printed Scaffolds. Small. 2024-Dec;20(50):e2405642. doi:10.1002/smll.202405642

Abstract

Carvacrol is a potent antimicrobial and anti-inflammatory agent, while curcumin possesses antioxidant, anti-inflammatory, and anticancer properties. These phytochemicals have poor solubility, bioavailability, and stability in their free form. Nanoencapsulation can reduce these limitations with enhanced translational capability. Integrating nanocarriers with 3D-printed calcium phosphate (CaP) scaffolds presents a novel strategy for bone regeneration. Carvacrol and curcumin-loaded nanoparticles (CC-NP) synthesized with melt emulsification produced negatively charged, monodispersed particles with a hydrodynamic diameter of ≈127 nm. Their release from the scaffold shows a biphasic release under physiological and acidic conditions. At pH 5.0, the CC-NP exhibits a 53% release of curcumin and nearly 100% release of carvacrol, compared to 19% and 36% from their respective drug solutions. At pH 7.4, ≈40% of curcumin and 76% of carvacrol releases, highlighting their pH-sensitive release mechanism. In vitro studies demonstrate a 1.4-fold increase in osteoblast cell viability with CC-NP treatment. CC-NP exhibit cytotoxic effects against osteosarcoma cells, reducing cell viability by ≈2.9-fold. The antibacterial efficacy of CC-NP evaluated against Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) exhibiting 98% antibacterial efficacy. This approach enhances therapeutic outcomes and minimizes the potential side effects associated with conventional treatments, paving the way for innovative applications in regenerative medicine.

Key Findings

This approach enhances therapeutic outcomes and minimizes the potential side effects associated with conventional treatments, paving the way for innovative applications in regenerative medicine.

Outcomes Measured

  • inflammatory markers

Population

Field Value
Population See abstract
Sample Size See abstract
Age Range See abstract
Condition See abstract

MeSH Terms

  • Cymenes
  • Nanoparticles
  • Tissue Scaffolds
  • Curcumin
  • Printing, Three-Dimensional
  • Humans
  • Monoterpenes
  • Cell Survival
  • Staphylococcus aureus
  • Osteoblasts
  • Bone Regeneration

Evidence Classification

  • Level: Other
  • Publication Types: Journal Article, Research Support, N.I.H., Extramural
  • Vertical: oregano-oil

Provenance

Source extracted via PubMed E-utilities API on 2026-04-12