Live long and active: Polypeptide-mediated assembly of antibody variable fragments

source extracted confidence: high 2026-04-09
#albumins #antibodies #biomedical-engineering #collagen #drug-delivery-systems

Live long and active: Polypeptide-mediated assembly of antibody variable fragments

Lee et al., 2020 | Adv Drug Deliv Rev | Systematic Review

Citation

Lee Changrim, Choi Minchang, MacKay J Andrew. Live long and active: Polypeptide-mediated assembly of antibody variable fragments. Adv Drug Deliv Rev. 2020-Dec;167:1-18. doi:10.1016/j.addr.2020.10.017

Abstract

Antibodies possess multiple biologically relevant features that have been engineered into new therapeutic formats. Two examples include the adaptable specificity of their variable (Fv) region and the extension of plasma circulation times through their crystallizable (Fc) region. Since the invention of the single chain variable fragment (scFv) in 1988, antibody variable regions have been re-engineered into a wide variety of multifunctional nanostructures. Among these strategies, peptide-mediated self-assembly of variable regions through heterologous expression has become a powerful method to produce homogenous, functional biomaterials. This manuscript reviews recent reports of antibody fragments assembled through fusion with peptides and proteins, including elastin-like polypeptides (ELPs), collagen-like polypeptides (CLPs), albumin, transmembrane proteins, leucine zippers, silk protein, and viruses. This review further discusses the current clinical status of engineered antibody fragments and challenges to overcome.

Key Findings

This review further discusses the current clinical status of engineered antibody fragments and challenges to overcome.

Outcomes Measured

  • Requires manual extraction

Population

Field Value
Population See abstract
Sample Size See abstract
Age Range See abstract
Condition See abstract

MeSH Terms

  • Albumins
  • Antibodies
  • Biomedical Engineering
  • Collagen
  • Drug Delivery Systems
  • Humans
  • Nanostructures
  • Peptides
  • Proteins
  • Single-Chain Antibodies
  • Viral Proteins

Evidence Classification

  • Level: Systematic Review
  • Publication Types: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Systematic Review
  • Vertical: collagen

Provenance

Source extracted via PubMed E-utilities API on 2026-04-09