Nicotinamide Riboside Augments the Aged Human Skeletal Muscle NAD+ Metabolome and Induces Transcriptomic and Anti-inflammatory Signatures

source extracted confidence: high 2026-04-09
#aged #aged-80-and-over #aging #anti-inflammatory-agents #cross-sectional-studies

Nicotinamide Riboside Augments the Aged Human Skeletal Muscle NAD+ Metabolome and Induces Transcriptomic and Anti-inflammatory Signatures

Elhassan et al., 2019 | Cell Rep | Rct

Citation

Elhassan Yasir S, Kluckova Katarina, ... Lavery Gareth G. Nicotinamide Riboside Augments the Aged Human Skeletal Muscle NAD+ Metabolome and Induces Transcriptomic and Anti-inflammatory Signatures. Cell Rep. 2019-Aug-13;28(7):1717-1728.e6. doi:10.1016/j.celrep.2019.07.043

Abstract

Nicotinamide adenine dinucleotide (NAD+) is modulated by conditions of metabolic stress and has been reported to decline with aging in preclinical models, but human data are sparse. Nicotinamide riboside (NR) supplementation ameliorates metabolic dysfunction in rodents. We aimed to establish whether oral NR supplementation in aged participants can increase the skeletal muscle NAD+ metabolome and if it can alter muscle mitochondrial bioenergetics. We supplemented 12 aged men with 1 g NR per day for 21 days in a placebo-controlled, randomized, double-blind, crossover trial. Targeted metabolomics showed that NR elevated the muscle NAD+ metabolome, evident by increased nicotinic acid adenine dinucleotide and nicotinamide clearance products. Muscle RNA sequencing revealed NR-mediated downregulation of energy metabolism and mitochondria pathways, without altering mitochondrial bioenergetics. NR also depressed levels of circulating inflammatory cytokines. Our data establish that oral NR is available to aged human muscle and identify anti-inflammatory effects of NR.

Key Findings

Our data establish that oral NR is available to aged human muscle and identify anti-inflammatory effects of NR.

Outcomes Measured

  • inflammatory markers

Population

Field Value
Population See abstract
Sample Size See abstract
Age Range See abstract
Condition stress

MeSH Terms

  • Aged
  • Aged, 80 and over
  • Aging
  • Anti-Inflammatory Agents
  • Cross-Sectional Studies
  • Cytokines
  • Double-Blind Method
  • Humans
  • Male
  • Metabolome
  • Muscle, Skeletal
  • NAD
  • Niacinamide
  • Pyridinium Compounds
  • Transcriptome

Evidence Classification

  • Level: Rct
  • Publication Types: Journal Article, Randomized Controlled Trial, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
  • Vertical: nicotinamide-riboside

Provenance

Source extracted via PubMed E-utilities API on 2026-04-10